Laboratory

Nitrate and Microfossil N isotope analysis

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"SigBench" coupled to MAT-253-Plus IRMS

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Clean Room

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Environmental Worker

Bacteria Lab

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Misty Slopes

NOx Box

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Nitrate and Microfossil N isotope analysis

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"SigBench" coupled to MAT-253-Plus IRMS

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Clean Room

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Environmental Worker

Bacteria Lab

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Misty Slopes

NOx Box

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Nanomolar-scale Nitrogen isotope Lab

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"SigBench" coupled to MAT253-Plus IRMS

The “Sigbench” is an automated system for the extraction and purification of bacterially produced N2O for the analysis of Nitrogen and Oxygen isotopes by isotope ratio mass spectrometry (Thermo MAT253 Plus). This system is used to perform nanomolar-scale high-precision measurements (within 0.1 ‰) of Nitrogen and Oxygen isotopes of nitrate and nitrite.

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Clean Room

In this specially designed clean room organic N is oxidized to Nitrate using a basic solution of Potassium Peroxydisulfate. The low procedural blanks allow us to perform nanomolar-scale high precision measurement of the 15N/14N ratio of organic N in different sample types including microfossils (e.g. foraminifera, diatoms and corals), tooth enamel, speleothems,  etc., using the denitrifier method.

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Bacteria Lab

In this lab we grow, culture and harvest two strains of denitrifying bacteria (Pseudomonas chlororaphis and Pseudomonas aureofaciens) used for isotopic measurements. The “denitrifier method” allows for nanomolar-scale high precision measurement of the 15N/14N and 18O/16O composition of nitrate dissolved in natural waters and the 15N/14N of other N forms (e.g., organic N) that can be converted into nitrate.

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"NOx Box"

The organic N content of different sample types is typically estimated prior to isotopic analysis by measuring the nitrate concentration after the organic matter oxidation step with Potassium Peroxydisulfate. The nitrate concentration is analyzed by reduction to nitric oxide using an acidic solution of vanadium(III) followed by chemiluminescence detection.

Biomarker Lab

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GC-Isolink-II coupled to MAT253-plus IRMS

This Thermo GC-IRMS system is used to de­term­ine the re­l­at­ive ra­tio of stable iso­topes of hy­dro­gen (2H/1H), car­bon (13C/12C) and nitrogen (15N/14N) in in­di­vidual com­pounds (e.g. long-chain n-alkanes, fatty acids, alkenones, amino acids, etc.)  of com­plex sample frac­tions or mix­tures at natural abundance concentrations.

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HPLC-Isolink coupled to Delta V IRMS

This Thermo LC-IRMS sys­tem is used to perform re­pro­du­cible and ac­cur­ate de­term­in­a­tions of stable car­bon iso­tope ra­tios of wa­ter-sol­uble or­ganic com­pounds with substantially lower sample amounts than those required by conventional elemental analyzers.

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GC-MS

This Agilent 7890B GC coupled to a 5977B single quadrupole mass spectrometer is used for the routine identification, characterization,  and quantification of organic compounds used in paleoenvironmental and paleoclimatic recconstructions

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HPLC-MS

This Agilent 1260 HPLC coupled to a 6130 single quadrupole mass spectrometer is mainly dedicated to the analysis of glycerol dialkyl glycerol tetraethers (GDGTs). The degree of cyclization of these group of  compounds produced by marine Thaumarchaeota can be used to reconstruct changes in the temperature of the ocean in the distant past. 

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GC-FID

This 7890B GC coupled to a Flame Ionization Detector is used for the routine quantification of know organic compounds used in for reconstruction of past climatic and environmental changes

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HPLC-DAD-ELSD with FC

This Agilent 1260 HPLC is coupled to a Diode Array Detector and an Evaporative Light Scattering Detector, as well as to a Fraction Collector. The system is used for the isolation of individual organic compounds for isotopic analysis

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ASE 350

This Accelerated Solvent Extractor is used for the simultaneous extraction and chromatographic separation of organic compounds used in paleoenvironmental reconstructions from marine and lake sediments and soils. 

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Rocket Evaporator

This Automated Evaporation System‎ is used to concentrate or dry large volumes of solvent for chromatographic analysis. The system is designed to maximize analyte and solvent recovery